THE NEOFLUIDICS PLATFORM TECHNOLOGY ALLOWS HIGH THROUGHPUT 2D AND 3D CELL CULTURE FOR DRUG DISCOVERY
Cell culture is one of the primary techniques used in cell and molecular biology, allowing scientists to grow cells in a controlled laboratory environment. Cell culture technology has not changed much in last half century. Advancements in microfluidic devices are making cell culture and experimenting in cell biology easier, quicker, and more efficient.
There are inherent advantages to using microfluidic devices for cell culture:
Easier to control and tailor the cell microenvironment while miniaturizing the experiments to the physical scale of biological cells.
Lower utilization of expensive reagents
Cell culture is commonly used in drug screening and development. Some researchers are content to perform in-vitro cell culture in two-dimensional formats. However, 3D cell culture can mimic the in vivo physical microenvironment better.
Microfluidic cell culture is vital to studying the effects of drugs and toxic compounds on cells. With the pharmaceutical industry constantly looking for more predictive screening models that resemble in vivo conditions, 3D cell culture has become a more valuable tool than its 2D counterpart. Neofluidics offers devices for 2D and 3D cell culture.
The NeoCULTURE™ product line includes Neo2D™ and Neo3D™ devices which have a unique, patented design that allows for on-chip trapping and culture of cells. This allows for easy culture and testing of drugs and chemicals. Features include:
Consistent number of cells can be trapped in each well based on the starting number
A consistent number of spheroids can reproducibly be generated in 3D culture
Media can be changed easily
On chip immunostaining
On chip FISH
Cells can be easily lysed and the lysate can be collected for off-chip analysis like ELISA on the NeoIMA™ devices
The Neo3D™ devices have been shown to predictably trap consistent numbers of cells in the wells. The number of cells trapped in a well are a function of the initial cell
population. The number of spheroids produced are also a function of the initial cell concentration and can be consistently reproduced.
The figure above shows how A549 lung adenocarcinoma cells were grown in 3D cell culture to generate spheroids which were then tested against Paclitaxel.
Product Name Catalog No.Neo2D™ (96 wells) NF004
Neo3D™ (96 wells) NF005